Lab Practical Overview. Mostly Finished.

Lab Practical Overview:

A. Polifera: Sponges
Q2: Drawing in lab review/notebook. Yes, the spicules are visible. Spicules are the structural support for sponges; they are stiff needles of silica or calcium carbonate.
Q3: They have pores and tubes and structures along with water canals. The water provides the nutrients and oxygen and carries out waste and carbon dioxide. They also have cells with different activities.
1. 4 elements: 0 # of tissues, asymmetrical, N/A, N/A
2. Location & fnx:
a. Tubes on the side are choanocytes (ostia) that move water from the outside to the center
b. osculum where water eventually exists; big opening at end

B. Cnidaria: Hydra, Jellyfish, Corals, Sea Anemones
1. 4 elements: 2 tissues, radial symmetry N/A, N/A
2. Location & Fnx:
a & b: Cnidocytes are specialized cells on tentacles that release stinging organelle called nematocyst (which have toxic barbs to hold prey and until brought to mouth for indigestion).
3. They eat using the process describes above ^


C. Rotifera: Rotifers
Q1: Corona = locomotion & wave food into mouth
Q2: The algae is eaten by the rotifer- it’s their diet plus phytoplankton
1. 4 elements: 3 tissues, bilateral, pseudocoelomate, protostomes
2. Cuticle covering: very close to arthropods and nematodes
3. Location & fnx:
i. Toe (cement glands (sticky) attach rotifer to objects in water)
ii. Corona (crown of celia) – wave food into the mouth, also propel themselves
iii. Celia – rapid movement makes it appear to whirl like a wheel
iv. Excretory system: Intestines


D. Platyhelminthes: Planaria (flat worms)
Q5: Single opening digestive tract visible
Q7: Acoelomate type of body cavity- meaning none
Q8: Protostomes: blastopore developing into the mouth
Q11: Sessile animals have radial symmetry, multidirectional feeding and movement. Motile animals have bilateral symmetry and directional movement to obtain food.
1. 4 elements: 3 tissues, bilateral, acoelomate, protostomes
2. Location & function:
a. a. Pharynx: dorsal part; towards the middle- muscular- has jaws called trophi which ground food- all rotifers have it
b. Flame cells: part of excretory system; move fluid through canals
c. Eyespot: dorsal surface between two auricles that are on the head end; pigmented cups with retinal cells; sensitive to light intensities but can’t form images



E. Annelida: Earthworm (segmented)
Q1: Function of the cuticle in earthworms is that they help with lomotion and since they have no skeleton, cuticle protect them with their bristles like feature
Q2: Advantage of segmentation: Locomotion- each segment has muscles that flex and push against rest of the body, causing the worm to move forward
1. 4 elements: 3 tissues, bilateral, eucoelomate, protostomes
2. Location: heart, crop where the esophagus ends, intestine
Excretory system: Nephridia: pair of tiny white coiled tubes in each segment



F. Mollusca: Clam/Mollusks
1. 4 elements: 3 tissues, bilateral, eucoelomates
2. 4 unifying features: Mantle, Visceral Mass, Foot
3. Location & fnx:
i. Gills: pleated appearance: use for breathing (act like lungs); trap food particles on their surface as water comes in, which is then moved to mouth by ciliary movements.
ii. Mantle: secrets calcium carbonate that produces the shell
iii. Adductor muscles: close the shell and hold it there; anterior and posterior; pink roundish with dots
iv. Foot: strong muscle used for locomotion
v. Incurrent/excurrent siphons: excretory system: incurrent is where water comes in and excurrent is where wastes and water leaves: both at the posterior end of clam…. Excurrent is more dorsal



Lab 13:

A. Nematoda: Nematodes/roundworms (type of Ecdysozoa)
1. 4 elements: 3 tissues, bilateral, pseudocoelum, prostotomes
2. Key characteristics: molting of the exoskeleton (cuticle made of chitin)
3. Morphological differences between male and female are that males are smaller and have a hook like tale they use during sex to hold females
4. Locate and fnx:
i. Uterus: 3rd down, below vagina/tested, in the middle
ii. Testes:
iii. Ovaries: 5th down, 3/4th way down, on top of rectum ***Remember that in nematodes there are two longitudinal tubes in the two lateral lines that are the excretory system: excrete nitrogenous wastes (but no flame cells like flat worms)
B. Arthropoda: Crayfish & Grosshopper:
1. 4 elements: 3 tissues, bilateral, eucoelumate, protostomes
2. 4 unifying characteristics:
3. Location and fnx on Crayfish:
i. Caraspace: expanded exoskeleton plate: feather gills under it
ii. Greenglands: excretory system: long tube-like structures compacted into glandular mass: waste and excess water pass through the pores from which antenna comes out of
4. Location and fnx on Grasshopper:
i. Spiracle: respiratory system: small openings into air tubes (trachea) which branch everywhere and bring oxygen to cells
ii. Malphigian tubules: excretory system: within them are mini tubes that empty into intestine: wastes and salts from blood
iii. Head: Upper part with compound eye, antennas, mouth, brain
iv. Thorax: Middle part: crop, gastric caeca, esophagus, heart,
v. Abdomen: spiracles, intestines, anus, ovary, rectum, ovipositor


Lab 14: Animal Diversity III – Deuterostomes (anus forming first, mouth 2nd)

A. Echinodermata: Sea Stars, sea cucumbers, sea urchins
A. Remember, two symmetry: Radial in adults and bilateral in larvae before metamorphosis.
B. Location and function:
i. Mouth: food/water enterance
ii. 2 stomachs: extending can take place, able to eat bigger stuff than mouth size. Cardiac first and then pyloric.
iii. madreporite: dorsal surface; acts as a water supply and filter
iv. tube feet: movement/feeding; part of vascular water system

B. Chordata: Lancelet, fish, frogs, pigs, humans
A. Four unifying characteristics:
i. Pharyngeal gill slits: openings in the throat
ii. Notochord: running the length of the body
iii. Dorsal hollow nerve cord: bundle of nerve cells running the length of the body as well
iv. A muscular tail – extending past the anus

1. Location and function:
a. Tentacles: for feeding (located on top of the mouth)
b. Mouth: place for water and food (trapped by mucus) to enter body
c. Pharyngeal gill slits: enclosed by the body wall folds, water goes through them and they end up forming a cavity called the atrium
d. Notochord: support along the body length
e. Nerve cord: sensory
f. Intestine: digestion system
g. Anus: place for the wastes to come out of
h. Nephridia: excretory system
Remember, evidence of segmentation: gills, muscles
Evidence of nervous system: nerve cord

2. Location and function:
a. Dorsal fin: movement
b. Nerve cord: sensory
c. Notochord: support

C. Chordata: Pig
1. Location and function:
a. lungs: part of respiratory system: exchange gases
b. heart: pumping blood through blood vessels
c. liver: detoxification, protein synthesis, and production of biochemicals necessary for digestion
d. kidneys: excretory system: reg. of electrolytes, acid-base balance, and blood pressure; excretion of wastes (urea and ammonium); reabsorption of glucose and amino acids; production of hormones (vitamin D and erythropoietin).
e. diaphragm: layer of muscular membrane bottom of thoracic cavity which adjust its volume
f. larynx: protection of trachea and production of sound
g. umbilical cord: connecting cord from the fetus or the embryo to the placenta: supplies oxygenated, nutritious blood


Lab 10 & 11: Animal Development

A. Egg Types:
a. Isolecithal – (same/yolk) – meaning eggs containing small amounts of evenly distributed yolk; impact of yolk is minimum
1. echinoderms, mollusks, and mammals
b. Strongly Telolecithal - (end/yolk) – meaning eggs contain large amounts of yolk concentrated at one end.
1. reptiles, birds, fish, chicken, etc.
2. nucleaus surrounded by active cytoplasm which is devoid of yolk and called blastodisc (towards animal pole where polar bodies budded from the cell during meiosis)
3. Rest of egg: vegetal hemisphere associated with vegetal pole
c. Mesolecithal – moderately telolecithal – some amphibian species

B. 1st Step: Cleavage Types: subsequent mitotic divisions

a. Holoblastic Cleavage: Isolethical eggs
1. The cell divisions pass through the entire fertilized cell
2. blastocoel forms in the middle of the blastula

b. Uneven Holoblastic Cleavage: Moderately Telolecithal:
1. Yolk will retard cytoplasmic divisions and affect cell sizes
2. Blastocoel develops in the animal hemisphere
3. Cells will be smaller and have less yolk compared to vegetal

c. Meroblastic Cleavage: Strongly telolecithal
1. Only active cytoplasm is divided during cleavage
2. produces blastoderm (cap of cells)
3. blastocoel forms between two layers of cells within blastoderm


d. Blastula: multicellular ball/disc that a zygote converts to from cleavage
e. Blastocoel: A cavity that forms within the ball of cells after blastula forms
f. Blastomeres: these are the cells of the blastula


C. Stage 2: Gastrulation: cell movement: blastula becomes gastrula
1. Three germ layers: endoderm (involution) ectoderm (surface cells) mesoderm (develops between the two)
2. Archenteron: new internal cavity that forms through involution, where surface cells go to the interior of the embryo lined by the endoderm: blastocoel disappears
3. Blastopore: what the archenteron opens to the outside through
1. protostomes: blastopore becomes the mouth
2. deuterostomes: blastopore becomes the anus


D. Stage 3: Neurulation: formation of dorsal hollow neural tube in chordates (frogs)
a. Notochord is formed from mesoderm cells
b. Neural plate (ectodermal cells above flattened)
c. neural groove: when the center of the plate sinks
d. neural folds: edges of the plate become elevated
e. neural tube: when folds fuse together; anterior becomes brain and posterior becomes nerve or spinal cord
f. notochord disappears

E. Organgenesis:
a. Organs forming from each tissue layer:
1. Ectoderm: skin, glands, nervous system
2. Mesoderm: muscles, skeleton, circulatory, reproductive and excretory system
1. In chordates, somites and notochord will form as well
3. Endoderm: digestive tract lining and organs: liver and lungs, pancreas, thyroid, thymus, etc


F. Sea Star Development:
a. Type of egg/cleavage: Holoblastic cleavage
b. Fast and slow back to polyspermy (multiple fertilization)
1. fast block: sperm fuses with egg, plasma membrane permeability changes  influx of sodium ions [Na+]; changes electric potential ; less permeable
2. slow block: when egg is fertilized, calcium ions release in the ER and spread as a wave, therefore cortical granules fuse with egg cell’s plasma membrane and release their contents to the exterior, causing FERTILIZATION ENVELOPE to rise and clear away extra sperm
c. Know what stages of development listed above look like in the sea star


G. Frog Development:
a. Type of egg/cleavage: uneven holoblastic (moderately telolecithal)
b. Know what stages of development listed above look like in the frog


H. Chicken Development:
a. Egg components:
1. shell membranes
2. albumin
3. chalaza
4. yolk – rich in protein and fat
5. air chamber
b. Embryogenesis in chickens is different from sea star and frog development because: it is through meroblastic cleavage due to being a strong telolecthical type of egg.

c. Know these terms relating to only chicken development:

1. blastodisc - active cytoplasm that is devoid of yolk
2. primitive streak
3. somites
4. vitelline blood vessels
5. 4 extra embryonic membranes


Regeneration:
Morphallixus: regeneration occurring through already present tissues in the organism via reorganization. The regenerated organism is smaller than parent. Examples? 2 Hydra smaller than parents.

Epimorphosis: regeneration occurring through cellular proliferation of stem cells, where the organism is the same size as the parent. Examples? Head in planaria, same size as parents.